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Module 16: Methods and Instruments


In this resource module students work on molecular separation and characterization using techniques similar to those used by professionals in the molecular biology labs. Though students work in the virtual simulated environment, they solve similar problems by, for example, placing a mixture of proteins into gel and exposing them to the to the external electric field. In addition, they can treat proteins with similar mobility with SDS thus increasing sensitivity of the PAGE. In another set of activities students explore principles of mass-spectrometry. They manipulate the strength of magnetic field and/or charge and mass of the particles and learn how the differences in the resulting trajectories (deflection angles) allow separating atoms and molecules. This module will include such techniques as chromatography, gel-filtration, sedimentation analysis etc.


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These materials are based upon work supported
by the National Science Foundation under grant numbers
9980620, ESI-0242701 and EIA-0219345

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